[Frontiers in Bioscience 11, 883-888, January 1, 2006] 883 Tet responsive and adenovirus based constructs for regulated in vivo expression of Lefty

نویسندگان

  • Anatoly Mikhailik
  • Meiyi Tang
  • Patrick Hearing
  • Viswanathan Ravishankar
  • Siamak Tabibzadeh
چکیده

1. Abstract 2. Intdocution 3. Methods 3.1. Materials 3.2. Isolation and culture of decidual cells 3.3. Digestion and purification of constructs 3.4. Transfection 3.5. Qauntitative PCR 3.6. SDS-Polyacrylamide Gel Electrophoresis and Western Blotting 3.7. β-gal staining 3.8. Statistical analysis 4. Results and discussion 4.1. Generation of Lefty-A tet on constructs 4.2. Construction and preparation of Ad-Lefty recombinant virus 5. Acknowledgement 6. References

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Tet responsive and adenovirus based constructs for regulated in vivo expression of Lefty.

Regulated expression of Lefty/Ebaf during embryogenesis is required for development of body asymmetry. A tight regulation of Lefty also contributes to the menstrual tissue shedding in humans. In order to replicate this regulated expression, we have developed a tet-on system and an adenovirus driven model. To drive the expression of Lefty, we have placed Lefty under control of a tetracycline-res...

متن کامل

USF-1 and USF-2 trans-repress IL-1beta-induced iNOS transcription in mesangial cells.

Transcriptional activation of the inducible nitric oxide synthase (iNOS) gene requires multiple interactions of cis elements and trans-acting factors. Previous in vivo footprinting studies (Goldring CE, Reveneau S, Algarte M, and Jeannin JF. Nucleic Acids Res 24: 1682-1687, 1996) of the murine iNOS gene demonstrated lipopolysaccharide-inducible protection of guanines in the region -904/-883, wh...

متن کامل

miR-506 inhibits cell proliferation and invasion by targeting TET family in colorectal cancer

Objective(s): Ten-eleven translocation (TET) family members have been shown to be involved in the development of many tumors. However, the biological role of the TET family and its mechanism of action in colorectal carcinogenesis and progression remain poorly understood. Materials and Methods:We measured the expression levels of TET family members in colorectal cancer (CRC) specimens, in the c...

متن کامل

Lefty peptides, derived by MMP2 cleavage, act as a new class of gelatinase A inhibitor.

MMPs are zinc-dependent endopeptidases that are involved in proteolysis of extracellular matrix in both physiological and pathological processes including cancer. MMPs are involved at all stages of tumor progression, including tumor growth, angiogenesis, and metastasis. We recently showed that overexpression of Lefty in cancer cells restrains tumor growth. Here, we show that small forms of Left...

متن کامل

The Impact of Authentic Leadership on Risk Perception of Employees with the Mediating Role of Safe Climate in Ilam Petrochemical Company

This study investigated the impact of of authentic leadership on risk perception of employees with the mediating role of safe climate in Ilam Petrochemical Company. The present study is considered a descriptive survey in terms of purpose, and applied in terms of data collection. The statistical population consisted of Ilam Petrochemical Company's official and contract staff with about 700 perso...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

عنوان ژورنال:

دوره   شماره 

صفحات  -

تاریخ انتشار 2005